Dr ZHANG Daohai

Assistant Professor (Adjunct), Dept of Pathology, National University of Singapore

Scientific Officer, Dept of Laboratory Medicine, National University Hospital

E-mail: patzd@nus.edu.sg; or daohai_zhang@nuh.com.sg

Homepage: http://www.zhangdaohai.page.tl

 

 

 

 

 

 

 

Research interests

Basic and translational research

  1. Biomarker/drug target discovery and validation in clinical specimens using proteomics, tissue microarray, protein array and bioinformatics
  2. Functional proteomics study of target genes/proteins by using siRNA, chemical inhibitors, cell culture, animal model, and other available techniques.
  3. HER-2/ neu -mediated cellular proteome, phosphoproteome and secreted proteome in both cancer cell line and tumors
  4. Role of CK19 expression in cell invasion and metastasis

Clinical service-based molecular diagnosis

  1. High-throughput HLA-genotyping for transplantation
  2. Development and application of diagnostic proteomics

Representative publications

•  Zhang D , Tai LK, Teh M, Sethi SK, Koay ESC (2006) Comparative proteomics of colorectal cancer: abnormal expression of proteins within the tumor nest and implication of HnRNP A1 as an oncoprotein in tumorigenesis . J Cancer Mol 2: 17-24.

•  Zhang D , Tai LK, Wong LL, Chiu LL, Sunil K Sethi, Koay ESC. (2005) Proteomics study reveals that proteins involved in metabolic and detoxification Pathways are highly expressed in HER-2/ neu breast cancer. Mol. Cell Proteomics 4: 1686-1696

•  Zhang D, Tai LK, Wong LL, Chiu LL, Sunil K Sethi, Koay ESC. (2005) Proteomics analysis reveals the enhanced e x pression of CK19 in HER-2/ neu positive breast tumors. Proteomics 5: 1797-1805.

•  Zhang D , Li N (co-author), Lok SM, Zhang LH and Swaminathan K. (2003). Isomaltulose Synthase (PalI) from Klebsiella sp.:Crystal Structure and implication of mechanism. J. Biol. Chem. 278 (37): 35428-35434.

•  Zhang D , Salto-Tellez M, Putti TC, Do E, and Koay ESC (2003) Reliability of tissue microarrays in detecting protein e x pression and gene amplification in breast cancer. Modern Pathology 16: 79-85.

•  Zhang D , Li N, Swaminathan K, Zhang LH (2003). A motif rich in charged residues determines product specificity in isomaltulose synthase. FEBS Lett 534:151-155.